Gentamicin exclusion assay in layman’s terms
Should be easy. :
So I need to do an experiment that kills bacteria on the outside of human airway cells whilst leaving the ones inside alive so I can quantify how many bugs got into the cells.
The antibiotic gentamicin will kill any bacteria outside of the human cells but cannot get into the cells thus leaving internalised, protected bacteria alive. The first thing I have to do is determine what concentration of the antibiotic will kill the bacteria on the outside. I do this simply by incubating various concentrations of the drug with a known number of bacteria on their own, wash the drug off after an hour and then see how many bugs grow on an agar plate overnight.
The problem is that I get different results when I wash the bugs before using them Vs not washing them and thus leaving them surrounded by lots of proteins (molecules/enzymes) that they had been making before I used them.
The eternal problem as a scientist is trying to figure out which condition is more relevant to either the physiological situation or the purpose for which you need the tool to work in the first place.
Should be easy. Never is ;)
My favourite restaurants
I have many memories, good and bad from various eateries in Edinburgh but here are a couple… :
Stac Polly – Discovered this place with Elissa’s dad. I actually have fond memories (makes a change) of being wooed here for my birthday one year with G spending an awful lot of money which made a refreshing change from me attending to the bill.
Kublai Khan – Where we had Elissa’s first birthday party. I have some very cool photos of me and Liss from that night. Unfortunately we went back later and it had gone rather downhill.
The witchery – I was always promised to be taken here but we never got round to it, is it worth it?
Vittoria’s – Definitely my favourite Italian in Edinburgh. Their steak balsamico is the best steak I have ever had! Thank you Stu for this one.
Sushiya – I had amazing sushi here with my friend Craig after I had just had General K. Managed to eat at the bar on a stool crosslegged with K feeding under a blanket = Supermum *grin*
I’ve decided that meals out in cheap places tend to be very ho hum (with a couple of exceptions)and not really worth the effort so it’s gonna be the slightly pricier ones from now on (on recommendation only) I have a few suggestions from work folk already. I also need to get Stu experimenting more so we can find new places :) he is too fussy and I’m sure we can find dishes in any restaurant he will eat ( I promise not to make you eat Curry of any description :P)